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Image Search Results
Journal: Toxicology mechanisms and methods
Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A
doi: 10.3109/15376516.2015.1070226
Figure Lengend Snippet: Effects of exposures to TBBPA on the phosphorylation/activation state of PKC and PKD in NK cells: Representative experiment for 10 min exposures to TBBPA. Experiment was repeated using cells from seven different donors.
Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of
Techniques: Activation Assay
Journal: Toxicology mechanisms and methods
Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A
doi: 10.3109/15376516.2015.1070226
Figure Lengend Snippet: Effects of incubations with K562 target cells on phosphorylation/activation state of PKC in NK cells: A) levels of phospho-PKC normalized to the control. B) Representative Western blot. Experiments were repeated using cells from five different donors. Asterisks indicate a significant difference as compared with control, p<0.05.
Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of
Techniques: Activation Assay, Western Blot
Journal: Toxicology mechanisms and methods
Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A
doi: 10.3109/15376516.2015.1070226
Figure Lengend Snippet: Effects of incubations with K562 target cells on phosphorylation/activation state of PKD in NK cells: A) levels of phospho-PKD normalized to the control. B) Representative Western blot. Experiments were repeated using cells from five different donors. Asterisks indicate a significant difference as compared with control, p<0.05.
Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of
Techniques: Activation Assay, Western Blot
Journal: Toxicology mechanisms and methods
Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A
doi: 10.3109/15376516.2015.1070226
Figure Lengend Snippet: Effects of exposures to TBT on the phosphorylation/activation state of PKC in NK cells: A) levels of phospho-PKC normalized to the control for 10 min exposures to TBT. B) Representative experiment for 10 min exposures to TBT. C) Representative experiment for 1 h exposures to TBT. D) Representative experiment for 6 h exposures to TBT. Experiments were repeated using cells from five different donors. Asterisks indicate a significant difference as compared with control, p<0.05.
Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of
Techniques: Activation Assay
Journal: Toxicology mechanisms and methods
Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A
doi: 10.3109/15376516.2015.1070226
Figure Lengend Snippet: Effects of exposures to TBT on the phosphorylation/activation state of PKD in NK cells: A) levels of phospho-PKD normalized to the control for 10 min exposures to TBT. B) Representative experiment for 10 min exposures to TBT. C) Representative experiment for 1 h exposures to TBT. D) Representative experiment for 6 h exposures to TBT. Experiments were repeated using cells from five different donors. Asterisks indicate a significant difference as compared with control, p<0.05.
Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of
Techniques: Activation Assay
Journal: Toxicology mechanisms and methods
Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A
doi: 10.3109/15376516.2015.1070226
Figure Lengend Snippet: Effects of exposures to DBT on the phosphorylation/activation state of PKC in NK cells: A) levels of phospho-PKC normalized to the control for 10 min exposures to DBT B) Representative experiment for 10 min exposures to DBT. C) levels of phospho-PKC normalized to the control for the 1 h exposures to DBT. D) Representative experiment for 1 h exposures to DBT. E) Representative experiment for 6 h exposures to DBT. Experiments were repeated using cells from six different donors for 10 min and 1h and form three different donors for 6 h. Asterisks indicate a significant difference as compared with control, p<0.05.
Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of
Techniques: Activation Assay
Journal: Toxicology mechanisms and methods
Article Title: Activation of Protein Kinase C and Protein Kinase D in Human Natural Killer Cells: Effects of Tributyltin, Dibutyltin, and Tetrabromobisphenol A
doi: 10.3109/15376516.2015.1070226
Figure Lengend Snippet: Effects of exposures to DBT on the phosphorylation/activation state of PKD in NK cells: A) levels of phospho-PKD normalized to the control for 10 min exposures to DBT. B) Representative experiment for 10 min exposures to DBT. C) Representative experiment for 1 h exposures to DBT. D) Representative experiment for 6 h exposures to DBT. Experiments were repeated using cells from five different donors (10 min), six different donors (1 h) and three different donors (6 h). Asterisks indicate a significant difference as compared with control, p<0.05.
Article Snippet: NK cells were purified by mixing 30–40 mL of buffy coat with 0.6 mL of
Techniques: Activation Assay
Journal: The Journal of Allergy and Clinical Immunology
Article Title: Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding
doi: 10.1016/j.jaci.2014.08.039
Figure Lengend Snippet: Cytolytic activity of polyclonal activated NK cells from the 3 representative patients, patient UPN440 with Chediak-Higashi syndrome, patient UPN539 with GS2 with pigment dilution, and patient UPN396 with GS2 without pigment dilution, in comparison with healthy control subjects (means ± SEMs) against the 221 B-EBV target cell line.
Article Snippet: Polyclonal NK cell populations were also obtained after
Techniques: Activity Assay, Comparison, Control